FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951958
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC2506.203153E-520.2009819
TAGCATG3101.5256173E-518.6242858
AGTGAGT3002.154154E-417.1733041
ATAACCC4051.5034764E-414.2556248
CCTTTAG5357.723309E-613.4895524
GTGAGTA3800.001502454513.2957052
CCCCAAT5401.0473281E-412.2666461
CCCAAAT6053.0134487E-410.9487431
CCCATAT8204.475549E-59.8731551
CCCCATT6050.00369675649.7322161
CCCATCA9101.3173831E-59.7054781
GTATAAC5950.00378856769.7034086
TATAACC7452.2622777E-49.6871287
CTTTAAT14205.686161E-99.3295611
CACTTGA6400.0058777969.1999851
TTAGCAT7200.00178362349.0211377
TCAGTGT6500.0078368358.8832852
CTTTAGC8558.959177E-48.440835
CCACGAT8759.2745E-48.4114151
CATTGGA9250.00160678837.95674321
Produced by FastQC (version 0.11.5)