FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628835
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGGA4452.8746034E-413.2050541
CCCAGTT4150.002552406612.3896811
CTAAAAT7251.1735219E-511.144611
GATTTAC5600.002292635410.3088135
ACTTTGC7051.2963582E-410.2357026
GTCAATC5650.002468267710.2175862
CCTCAAA6505.9458474E-410.1704311
CAAAGCG7150.00142393239.2458471
AAGATTT11452.8737886E-68.8232633
AGATTTC7950.0043749348.1692494
CAACATT8400.00611869667.86997651