FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715994
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAT2450.001214591217.7591721
ATTGGGT3100.00595954313.9903398
CTCACCT3750.001311783713.5364351
AAGACTT6003.2959576E-410.8425124
CTTTAAA8355.6412664E-610.421551
ATTAAGA7201.5267404E-410.07175351
CTCCTCT5800.002945911810.0022931
CGATGAT11201.912831E-79.7120481
CTCAAAA10205.993177E-69.2423151
TCAACAC6450.00725118868.9672652
CTTGTAA6500.00753680948.9251231
CTTTCTT16359.622454E-108.8705341
CTTCGGT7600.0027877898.5874941
CTTTGTT10155.677344E-58.5733941
TATGATT8000.00455228848.133592
CACAAAA10004.5357138E-47.9768281
ATCAACG8250.00584266067.91090441
TTCGTTC8300.00633707647.8396052
CTTTGAA11201.7865699E-47.7696381
CTCTTTT14054.983336E-67.74198771
Produced by FastQC (version 0.11.5)