FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944718
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATCC4904.9829592E-513.27951051
CCCAGAA6900.00119005289.4303771
CAATTCA12005.6081753E-68.3978944
CTCAAAA15001.3123015E-57.22995571
CTTTGTC14204.9750517E-57.1281251
CAAACAG13852.8292395E-46.786241
TAAGATT10750.0086112166.6988322
TCAATTC14153.878052E-46.61318253
GTTTTGT12000.00339940036.59834536
TTTGAAC12150.00385437536.5196452
CTTCAAA19007.6107517E-66.4689081
CTTTTGT17457.213588E-45.8005381
CGAAAAA17608.045508E-45.7511011
TAAATTT16800.00305294625.5700323
TTCAATT16900.00325881885.5394192
CTTTTAT20902.0804505E-45.53489451
CTTTCAT21352.8195133E-45.4182341
CTTTCAA20107.347884E-45.3954891
CAAAGAT18800.0018552785.384011
CTTTGAA17550.00485346145.3555231