FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1540147
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCATG4951.0175427E-817.5175518
TAACCCC5501.4762343E-411.82434759
CTCGGAT6253.5605502E-511.6257491
AGTGAGT6350.00612805369.1541331
GTGAGTA6350.00638251249.10893252
CCCGTAG7400.00215269048.837141
CCTTTAG11202.0464908E-58.3878554
ATAACCC9900.00374882857.29897988
CTCCGAT11300.00163714117.07318741
GTCATTT15251.1895022E-46.67051121
TATAACC17057.895099E-56.35717637
TCTCAAT13650.00178763646.35251867
CTTTAGC17708.714189E-45.71584375
GTATAAC15200.00594247235.70472966
AGCATGA16650.00262563565.6419159
GTTCCTT24054.410211E-55.43823961
GGTAAAT20100.0040304645.060961
Produced by FastQC (version 0.11.5)