FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695221
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2005.456968E-1239.3350457
ACACGTC3101.0040822E-925.3792748
CACGTCT3201.4497346E-924.5861749
GCACACG4756.9085218E-918.0703726
AGAGCAC5757.2761395E-814.9276993
GAGCACA6051.3558383E-714.1874834
AGCACAC5901.5807727E-613.3358335
AGTCACG1801.6733249E-48.75119220-24
GGTATTC7950.0045952278.1250421
GAAGAGC10851.3935924E-47.93782951
AAGAGCA10559.226365E-47.4643972
GTCACGT2600.0082820126.058517520-24
ACTCCAG3854.7707403E-45.572851715-19
ACGTCAT4408.448465E-55.53038925-29
ACGTCTG4007.967798E-45.36425610-14
CACGTCA3550.00626000675.241727425-29