FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences811998
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAACC4200.00308129912.0830091
CTCCCAA4650.006585410.9136851
CTCCGGA7652.8193355E-49.4768691
CCTCCGT6450.0070907548.9920061
CTCTGTT7400.00219468388.817331
CTCAATA6600.008561268.78764251
CTTTAAA11201.961041E-58.4149521
CTTAAAA13552.9895891E-68.0256151
GAATCTT8650.0093630727.5050584
CTTTGTT11000.0012587977.24980451
CTTCAAT10450.00613502556.93761251
ATTTAAT14304.0396178E-46.5907321
CAGAAAG13250.00122899226.5658611
CTCAAAA12650.00547076676.30417781
CTCTTTT17501.0774763E-46.2141181
GGAAGAA20502.3558749E-56.01203351
CTTTCAG14500.00338646865.99983931
CTTCTTT24802.0225434E-65.8466171
AAATTTA18100.0011822385.57926654
CAAATAT18750.00184618885.3858523
Produced by FastQC (version 0.11.5)