FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550474
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACACA4050.00287928412.1917489
CAACAGT4858.1874983E-411.6616121
CGAAAGA5150.001352941510.9822931
CTTCGTG4600.007319933710.758471
CTTCTAT7954.9440343E-59.7821861
ACGATAT6000.00487850239.4093363
CTATATT7050.00177358499.0253421
CTTTAAA8901.6940184E-48.7380181
CTTTTGT9352.8885528E-48.3174721
TTCTATT8500.00105287338.3031092
CTTTTAT11252.8368166E-58.1696061
AAGATAT12952.0183872E-57.6291923
TTTTGTT14701.38189425E-57.2016772
CAATAAA10800.00135077617.20077471
ATGATTT12050.0043598876.44207673
CTTTCTT14200.00354433785.97452261
TTTTATT17850.00130373725.53540662
Produced by FastQC (version 0.11.5)