FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755522
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA16054.2251704E-67.2259421
CTAAAAT19451.9658182E-77.08081961
TCGCATT12150.00370400866.5452352
CTCCGAT13450.00146156896.46708441
TCCGCCC17055.3766917E-45.93625452
GAGAATT15950.00156495365.89239032
CCCCGCC17557.5229775E-45.7822981
GTTCCGT17557.5229775E-45.7822981
CAAAAGT22207.981572E-55.5506711
TTCAACC15800.0091676395.484983
CACCAAC17300.00398284485.4468681
GTTTGAA20650.00102094415.2652711
GAATTTA22405.6692003E-45.15849354
CAAACTC18600.0097164685.04755833
Produced by FastQC (version 0.11.5)