FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846976
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3353.6379788E-1227.5082747
CACGTCT3505.456968E-1226.3215779
ACACGTC4405.456968E-1222.5548618
GAGCACA4457.2759576E-1222.3014344
AGAGCAC4601.0913936E-1121.5742153
GCACACG5007.3123374E-1018.4305446
AGCACAC5301.5897967E-917.3873065
CTCAGCT4050.00240336812.4902631
CTCTTGT4856.818202E-411.9200161
ACGCATT6200.00660137110.909484145
AAGAGCA12258.458301E-1010.4172362
CCCATTT7853.3871092E-510.1263191
CCCCTGC6557.4171426E-49.9295551
CTCAAAG8751.0272492E-59.9106431
CCCAGAT6000.00401450659.6353451
GAAGAGC10105.53091E-69.3014481
AGTCACT2756.200935E-99.28574720-24
CCCGCTT8105.160367E-48.9216171
CTCCAAA9703.3948995E-48.1950111
CTTAAAA10650.00439218258.16565145
Produced by FastQC (version 0.11.5)