FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785099
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC3852.8040085E-718.5422558
CACGTCT4054.7710273E-717.6265899
GAGCACA4105.4220436E-717.4127394
CACACGT3555.3615822E-516.0873647
AGAGCAC4051.6476834E-414.1021693
AGCACAC5105.267593E-613.9975855
GCACACG4855.0961222E-513.2471996
GAAGGCC5953.4080847E-410.8036412
GAAGACG8200.00634119577.8392282
GAAGACA8550.0092527627.51497174
CTTTCAT14907.484713E-46.26396231
Produced by FastQC (version 0.11.5)