FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520687
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCCC1100.00987786526.2473967
GCTCCAG3650.001107177813.8428057
CGGTTTA3150.006681309513.7499574
CATCGAA5950.00367208439.7386051
CTCCTCC6759.557094E-49.657451
CTAAAAT7900.00399429448.2516181
CAAAGAT8850.0012140558.1842791
TTCGTTT9250.0019392637.804782
CTAAAAA10200.00488664267.1010661
CTCTTTT13750.00188294256.32123951
CTTCTTT19708.625623E-55.88271051
TAAATAA15750.008863955.4999833
CTTTTTC25159.335936E-55.18391941
TTATATT20050.00421480285.04099272
Produced by FastQC (version 0.11.5)