FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361582
Sequences flagged as poor quality0
Sequence length100-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1052.2463175E-433.4059037
ACACGTC1256.2405475E-428.0609598
CACGTCT1550.002187278422.6298079
AGAGCAC1850.006096888318.9601063
CCTCCAT2650.002500822815.9386661
CACCGCC3200.00895083113.1535749
CATAATA7000.00172553279.0508861
CTTAAAA6500.0095914998.6640971
Produced by FastQC (version 0.11.5)