FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673360
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGA2850.003234716115.3379011
TCTCCCC3700.001226430213.6574947
CTTCAAT11452.5260033E-68.9080391
CTCAAAA10155.37441E-58.6134021
CACAAAA10801.10942856E-48.0950031
CATTATT10809.824119E-47.4204191
CTTCAAA14455.6503843E-57.0586181
CTTTAAA10800.0080419926.7458361
TCAAATA11800.00275991046.730043
CAATATT14103.5777348E-46.65627434
CATCAAT12200.00355863016.56889631
CATCAAA14650.00359338225.9676471
TTTAAAA18552.52932E-45.83786872
Produced by FastQC (version 0.11.5)