FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813939
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTAC7950.0043452188.1760175
CTTCAAG10050.00357972167.33262631
CTTTCAG12404.7951264E-47.13157031
TTTAAAG13552.1912673E-46.9289987
TCAATTT14053.411094E-46.68282462
CTTGGAA11050.0089858466.669041
CAAAGAT14654.4434844E-46.53930141
CTTCTAT12850.00543310076.3083411
ATTAAAT16452.6431112E-46.2717351
ATTTTGC15150.00575428045.7205136
CTTTTTA16050.0087083385.50974941