FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974237
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3403.6379788E-1227.2233737
ACACGTC4405.456968E-1222.6544158
CACGTCT4405.456968E-1222.6544159
GCACACG5901.8189894E-1118.1015936
AGCACAC6359.731593E-1015.6975495
AGAGCAC5906.217306E-915.6896573
GAGCACA6702.07001E-914.8805834
AAGAGCA11453.4259756E-79.3312892
GAAGAGC11503.5421363E-79.3098421
CAAGCCA8350.0075079597.69316241
CTTTAAA13100.0092449365.99335861
CATATTT17800.00109406615.6137991
CACACAT4851.17216805E-55.57881125-29
Produced by FastQC (version 0.11.5)