FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577189
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1659.050564E-833.5515567
ACACGTC2251.3651679E-624.6044738
CACGTCT2751.7729304E-417.6145679
GCACACG3307.087482E-414.6838946
AGCACAC3459.92249E-414.0454635
GAGCACA4450.006688143610.8901234
GAAGAGC7850.0056107087.9461581
Produced by FastQC (version 0.11.5)