FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1410356
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCT6105.2536355E-410.3099846
GTCAACG9202.711115E-48.368721
AGAAGTC10650.00132108547.21778255
GATCCAT10450.0086290996.6978891
CAACGAC10550.0094588296.63440131
ACAAAGT16405.046146E-45.9654733
CCCGCAG17209.084104E-45.6970991
CAGGAAT21856.20452E-45.1253411
CAATGAA19150.0035135075.11697671