FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2567481
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCAT15854.7130015E-98.7419211
CTCTGAG9200.00167017747.9267421
CCCCGAG8550.0076642037.67642351
CTCAGGA17352.0022708E-77.5658131
CTCCGAT16759.2365553E-77.40144731
CTCATGG18851.0226722E-77.35063361
CTAAAAC13001.17098796E-47.29260251
CTCTCAT14154.2496144E-57.2152961
CTCATGT12204.4982915E-47.17305141
CTCAGGT11200.0014346697.1623781
CTCTTGT16002.8806395E-56.8368151
CTCACGG17109.91249E-66.82348781
CTCAAAT20353.8931466E-76.80881831
CTCAAGC18253.564246E-66.7931091
TTAGCCA11700.00259057856.77244047
TCCCCCG11800.00283296576.71504649
CTCGTGG13350.00125496216.55514861
CCCACAC13350.00125496216.55514861
CTTTGTT31457.2759576E-116.49261951
CTCAAAG23601.1035809E-76.489181
Produced by FastQC (version 0.11.5)