FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986958
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACCG6759.298889E-49.6881341
CTCAAAT7803.3510037E-49.3155151
CCCAGCG8054.595163E-49.0262131
CTCAGAA6500.00741895338.9428941
CTCCGAA9954.3973916E-58.7631371
CCCAGCT8800.00111458318.2569331
CTTTAAT12657.081368E-46.8927441
CTTTGAT13950.00214284756.25040961
TTCGTCA12800.0067013366.18261243
AAAGTTC13050.0081969526.0635575
CATAAAT14650.0037067495.9517551
CTTCAAA19303.9762008E-45.6472291
GTTTTCC18350.0086771645.0962726
Produced by FastQC (version 0.11.5)