FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1986183
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAGA11402.2103984E-47.62971971
GTCACAT11652.838926E-47.46599251
GTCAAGA13050.00103914926.6650431
GTGTGAA13200.0085410776.0401951
CACAAGA14850.0044369765.85715871
CTCCGAA18953.2153854E-45.73738861
CTCCAAC16950.00313015845.55911731
CTCCAAT15650.0079310975.55775171
AATCTTG18300.00137765145.51340875
ATCAACT18750.00174139535.41201451
CACAAAT17950.00614927945.2494171
CTCAAAG18250.00746499235.16312551
CTGTAAT18550.00902807255.07962471