FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753372
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACTA4703.8071466E-513.6646374
CTCACAA3250.00853773913.2506341
GACATGT3850.001805599212.97450355
CTCTCGT4000.00230393812.5604971
CCCGATT4000.00230393812.5604971
CTCCCCA4100.00277281512.2541441
CTCAAAG8304.8725815E-711.2417531
CCCGCTT6405.132285E-511.214731
CTCCAAC5250.001401432810.9370321
CTTAAAA7803.401783E-510.1220131
CTCAAAC6250.00594359739.1871061
CTTATAA7100.00164993579.0981461
CTTCAAT11202.2950735E-68.9717841
ATAAGGT6450.008081058.8496498
CTCATCT6500.0085993348.7827423
CTCAAGG7800.0038696418.2816471
CTATCAT8000.00486102568.0746051
AAAAGGG8250.00674993967.7847036
CTTTTAT12005.2692514E-57.77554561
TCAATTC10000.0046648837.13597733