FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107980
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAT4654.0823617E-412.6695721
CCCAAAT8103.2670578E-610.9099091
CCCAAAG9903.0053707E-710.4140031
AAACTCG5800.0030433249.963995
CCTCAAT7350.00179083569.0173731
CCTCCAC8557.3322E-48.6130861
CTTTAAA16707.522085E-77.49647951
CTTCAAT20404.4721673E-87.21979241
CTTCAAG16454.808144E-67.1627361
AAGTTGT11100.00144594147.1562289
CTTTGAA18605.4128395E-77.1266341
CTTTTGG12806.953799E-46.9039271
CATTGGA13109.0521394E-46.7458211
TCAAAGG12650.00567499736.2824972
ATCAGAT15750.00107679876.07837771
CAAAGGT14350.00314108246.0408863
CTATTAT13500.0091350826.00044971
CTTTAAT16400.00174834855.83746621
CATCAAA22256.48562E-55.62657071
CTTATCA17050.00277702025.6149241
Produced by FastQC (version 0.11.5)