FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977857
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATT7251.699121E-49.9664062
GTTAAAG6250.005072639.366191
CTCCAAT7903.5483335E-49.2624491
CCAGAAT10801.1041777E-58.8079031
CTTCAAT13352.258821E-78.769841
CATACCT6750.0095406778.6723981
CTACAAT7650.002728218.608631
CTTCGTT8650.0082410527.6134121
TCAGATT10900.0011843337.2919352
CTTTCTT21451.6638296E-87.16382461
ATTCTGA10250.00524805357.0504916
CAGATTT13750.00193637596.30698443
TTTTGCA23005.576185E-76.2841337
CGAAGAT13000.0066008096.19159171
CAATTTC13150.0084579746.04519274
TTTTGCG14750.00424779675.8793937
CTTCTTT22851.736332E-55.7642031
CTCTTTT17150.00320899145.54666851
TAAATAT17400.0044105335.3992983
Produced by FastQC (version 0.11.5)