FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688790
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGGG4057.636409E-616.1956671
GTTATGC4100.002471921312.4430121
CTCTTGT4600.00584170611.0905111
CTCCCAT4650.00633101210.9712591
GTTCATA7351.4903928E-510.9072871
CAACAAA11000.00118972077.288051
TTCATAT14000.00238427736.19058942
Produced by FastQC (version 0.11.5)