FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643251
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT3200.035.9621669
CACACGT3250.035.40897
ACACGTC3450.033.356218
GCACACG3850.031.7587936
GAGCACA4850.025.210594
AGCACAC4800.023.9747775
AGAGCAC4850.023.729463
GAAGAGC7750.016.7911471
AAGAGCA10957.2759576E-1212.4839022
AGTCACG2551.2532837E-910.18744120-24
GTCACGT2601.8595529E-89.43644220-24
CTCCACC8800.00117029628.2153651
CAGTCAC3351.5275873E-88.18543120-24
TCACGTC3758.9153946E-76.916994625-29
ACGTCTG3703.863709E-56.22048310-14
TCGTATG4953.379464E-76.11502940-44
ACTCCAG4257.134764E-66.09241415-19
TCTCGTA4052.1545937E-56.04909435-39
CCAGTCA4308.176474E-66.041389520-24
CACGTCA4102.6028229E-55.975045725-29
Produced by FastQC (version 0.11.5)