FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960379
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATGA6453.3645229E-612.4685491
CCCATAC6052.3941642E-512.084471
CTAAAAG5451.21467005E-412.0733831
CTCTATT6807.886208E-510.7516241
CCTACAT4950.00983982910.3389351
CCCACCT6200.00478106139.4336831
CCCATAT7752.9528225E-49.4336821
CTCCATT10951.3164517E-69.347531
CTCAAAA11753.4238583E-68.7111031
CTCCCAC8557.876628E-48.5509991
CTCTCCT8709.361111E-48.4035681
CTCATTT10457.262961E-58.3955261
CCCTTTT15201.8789979E-78.1768931
ATCCTCG8950.00140257798.0671732
CTTCAAG11853.5812867E-58.020621
CCCGCTT9200.00162667287.9468521
CTTTTGG10950.0088891936.67680741
CAAGAAG13250.00111778766.6213771
CCTTTGT12300.00374039616.5383861
CTCCAGA12700.0052240466.3324531
Produced by FastQC (version 0.11.5)