FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2622462
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT10050.014.025457
CACGTCT10200.013.818939
ACACGTC10203.8198777E-1112.4372748
AGAGCAC11101.2732926E-1112.0665423
GCACACG13555.12955E-109.8847696
GAGCACA13555.12955E-109.8847694
AGCACAC15606.82121E-109.0376935
CCCAAAT19151.7203784E-66.64660361
CCCATTT28803.7503924E-85.8927071
GAAGAGC26953.8389953E-75.7724471
CCCAGCC17258.285384E-45.7389831
CGCACAT15000.00652599435.6569981
CTTCGCG15200.00755359655.5825641
CTTCGAG15500.0093668495.4745141
CACAGAT28054.8605343E-65.29398251
CCCAGAT20500.00128954435.17408371
AAGAGCA31501.4699617E-65.14767272
CCCATAC19450.00375843475.08984381
Produced by FastQC (version 0.11.5)