FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences946066
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATT6053.723445E-410.7011741
CCCCCCC1304.4697535E-410.493322135-139
GTCAGTT7000.0014211389.2488721
CCCGAAT6450.0075604548.9222551
CTCAAGG6550.0085762718.78603651
CTTAAAT7600.00300017748.5186981
CTTTAAG7700.00337660248.4080651
CTCTGAA8550.0086503437.5721761
CTAAAAT8600.0091125647.5281511
CTTTAAT13753.476371E-57.32435941
CCTCCAT10850.00118234887.2930181
GAATGTT9850.0039104257.26682764
CTCATCC10250.0054874827.0181141
CATTTAT14356.105158E-57.0181141
CTTCTAT11350.00190523596.9717391
CTCAAAA13400.0015249886.44200041
CTTCAAA19108.929286E-66.4026521
CTATTAT12450.005027786.3557621
GAATTTC13650.00198659116.2925944
CTTCTCC16152.8482667E-46.23590951
Produced by FastQC (version 0.11.5)