FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782152
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAG3300.009205813.1004425
CCCGCTT4704.8411544E-412.4150661
CTCATGG4906.873661E-411.9083281
CCCAAGA5058.8524615E-411.5546151
CCTCGAA4550.00535440111.221311
CTTCTAA5450.001673915710.7065711
CTCAACA8255.648157E-48.8410321
CATCCAT12609.033032E-68.10427951
CTTCAAT13552.7414862E-68.0743741
CACAGAT8550.00764042377.6777381
CCTTCAT12456.81932E-57.616071
CATAAAG9850.00325227327.40492531
CCTTCTG10300.0050267647.0814091
CATTTTC21903.32293E-86.9091375
CAACAAC14103.1592158E-46.7248281
CTTTTAA14604.8299145E-46.49452541
CTTCTGT15308.514881E-46.197391
CTTCTTT24802.7808892E-76.1762451
TCAAATT14050.00255334986.1531572
TTCAAGG12900.00715609556.14322042
Produced by FastQC (version 0.11.5)