FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501887
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAG1700.003012912821.4173681
TTTAACA4250.0034655111.8933517
CTGGAAG9151.4166162E-610.3458761
CTTTGAT7953.9593427E-49.1596291
CTTCCGG9552.637182E-48.3875351
CTTCAAT8050.0045089178.141261
CTTTTTA10305.9461454E-47.77679161
TTTTTAA9900.00376005337.2946285
Produced by FastQC (version 0.11.5)