FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1475564
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAT9450.00220673537.7049311
CCCACAA11903.4386327E-47.3423461
CTCTTCT17301.5750193E-67.15489671
CTTGTAT15952.8149518E-56.84748551
CTTTGAT22655.1986717E-86.75074431
CTCAAAT13600.00157407926.42455241
CAATTCT14806.782246E-46.31627233
CTCCATT18101.6164062E-46.034111
CTTCAAG19909.308292E-55.8541991
TTTGAGA15150.00604698855.69570976
TTGGATT18550.0016826925.4264922
CTTTAAA23101.3570988E-45.3584291
CTTTTAT31658.502375E-75.2912061
CTCAAAA20050.00380757995.08410171
CATCCAT21750.00191569375.0214891
Produced by FastQC (version 0.11.5)