FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481448
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAT4201.0953208E-515.58943751
CCCAGCT4001.2614326E-414.550141
CTTTATT9156.85759E-1012.7214351
CCCATGC4050.0022830612.5741961
CAACCTT4650.006410737510.9517191
CAACAAG6658.0024253E-49.8459611
CTTTTAT9452.3542885E-59.2381851
CTTATTT6550.00780836968.8855831
GAAATAC9600.0028038337.51679855
TTTTATT18700.001770625.4024482
CTTTTTC19000.00195273235.3605781
Produced by FastQC (version 0.11.5)