FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521295
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACCATTCGGTATCTCGTATGCCGT7360.1411868519744099TruSeq Adapter, Index 9 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC3450.044.783438
CACGTCT3300.044.69099
CACACGT3350.044.0238727
GCACACG4400.039.9025886
AGCACAC4600.033.587575
AGAGCAC4950.032.6314543
GAGCACA6350.025.4371154
AAGAGCA9450.017.0943072
GAAGAGC9600.016.160711
CAGTCAC3500.010.041920-24
CTCGTAT3950.09.96371835-39
ACGTCTG3400.09.91366910-14
TCTCGTA4100.09.59919335-39
CATTCGG4250.09.58983625-29
AGTCACC3551.8189894E-129.50444620-24
ACTCCAG4500.08.73871515-19
CCATTCG4103.6379788E-128.56955625-29
ATTCGGT4605.456968E-127.94284330-34
CTGAACT5701.8189894E-127.14537810-14
TCTGAAC5504.9112714E-116.89450610-14
Produced by FastQC (version 0.11.5)