FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3552288
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGCT8801.17605305E-49.0398541
CTCAGAG9452.5471777E-48.4180641
CTCAAAT22751.6370905E-117.94712451
CCTAAAG12801.2550232E-57.9098721
CTCTCGT13954.7052345E-67.77621841
CCTACAG13051.624385E-57.7583421
CTTTAAA34200.06.97813221
CTCAAAC25953.45608E-106.9671321
CCCAAAC21101.1614975E-76.85486561
CTTAAAT19301.352284E-66.74476151
CTCCTAT21601.7788989E-76.6961881
CCCAGGG14605.3752546E-46.4393481
CCTCGAT35507.2759576E-126.3151651
CTCACGA14906.877236E-46.30969671
CACACAT16102.562395E-46.28859421
CTCACAT13900.00217477586.2433521
CTCAACG23508.145562E-76.15479371
ATCTAAA14150.00275236326.11360266
CTCAGCT17751.3600153E-46.111451
CACAAGA21407.319275E-66.08289241
Produced by FastQC (version 0.11.5)