FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906933
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2655.5388227E-926.8620577
ACACGTC3305.691072E-821.5710458
CACGTCT3459.1033144E-820.6331739
GCACACG4859.502401E-917.6126466
GTTACAC3050.00572355214.0764481
GAGCACA5801.3782974E-613.5004994
AGCACAC6303.485704E-612.429035
AGAGCAC5201.00433885E-412.3217443
GTCTAAA6100.00499201059.3842991
Produced by FastQC (version 0.11.5)