FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences967298
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGA6152.8332637E-511.8873861
CTCACCT4450.00446050211.5000451
CTTAAAC5350.001407492710.93195151
CTTCAGA10255.7603083E-69.2721611
CTTTCTA8305.865315E-48.8081231
CTTCGAT10951.329157E-58.67941951
CCCTGAT7900.00367869048.3286941
CATAGAT8250.0054354947.9753551
TTCGATA11051.611327E-47.83994532
GTTCAGG9500.00223166527.6955181
CCTGAAT8550.0074871857.6955181
GGGCATG8700.0097728977.4693826
CAATCAT11350.00160618697.0853011
CTTCCTT15702.1294592E-56.98478561
CTTTTAA17802.3089924E-66.98216961
GAACGAA10550.00700076536.8439835
GTTTCAT11800.00242104386.8150991
GTTTGTT11950.00276498736.7295541
CTTTAAT14153.2095838E-46.71658331
CATTGTT11050.00970779456.61605641
Produced by FastQC (version 0.11.5)