FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518966
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAT2900.003747050915.0018921
CTCAAAG4703.2717842E-513.884731
CTTCGAT5005.8515674E-513.0516461
CTTTAAG4200.003075188712.0848581
CTTAAAA6705.6292483E-611.9044871
CTTCCGT5651.8319012E-411.550131
CTTCTCA4600.006064696711.0340011
TTAAATG6250.00561513849.2498372
CTTTTAT12105.1359086E-57.79023841
TGTTTTC12054.3233566E-47.1950614
ATATTGT10250.00525022257.04882056
AATATTG10400.00604514826.94715455
CTTCTTC17850.0057092455.28077841
Produced by FastQC (version 0.11.5)