FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524201
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACT400.00613672954.178098
CTTAAAC3254.5073553E-415.5855961
CTTCCTC7402.054068E-49.7786071
CTTCCAT7402.054068E-49.7786071
CTTCAAA9050.00152833477.9957671
CAAAGAA9700.00302111477.45996861
CTCTTTT11250.00162669417.07536551
CTTTTTT17809.160643E-45.6913691
TTTATTT16750.00281496375.60702372
Produced by FastQC (version 0.11.5)