FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424563
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACACAGCAACATCTCGTATGCCGT6450.15192091633043858TruSeq Adapter, Index 5 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT2750.043.423369
ACACGTC2650.042.411278
CACACGT2700.041.625887
GAGCACA2850.039.4443324
AGAGCAC2950.038.107243
GCACACG3400.035.1218346
AGCACAC3450.032.5767755
AAGAGCA5350.021.0173532
GAAGAGC5650.020.0027351
CGCGGTG2000.00948887317.5609158
AACAAGT4650.00831537810.5743166
GTCACAC2406.7666406E-1010.5544520-24
AGTCACA2602.7465285E-78.66006220-24
TCTCGTA2355.7532234E-68.38347935-39
CTCGTAT2704.882204E-78.33912235-39
TGAACTC3753.1468517E-108.24192415-19
CAGTCAC2851.1071606E-67.900406420-24
ACGTCTG3601.0641088E-87.80485210-14
TCACACA3155.0099225E-67.1441125-29
ACACAGC4151.4231773E-76.77829825-29
Produced by FastQC (version 0.11.5)