FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3817428
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC13002.0086554E-78.8398298
CACACGT12901.7004531E-68.35158357
CTCAAGA25950.08.1803171
CACGTCT14609.9063345E-67.37768849
CTCCAAG27751.6552804E-106.85835271
CCCACGA16403.858877E-56.69509651
CTTGGTT25104.863432E-86.41591261
CCCACAT27657.576091E-96.35368351
CTCACTC16352.6742977E-46.26786661
CCCAAAT31755.7298166E-106.2248591
GAGCACA21609.511301E-65.9853014
GCACACG19659.465587E-55.84823276
CCCAGAT30757.203016E-85.71314951
CTCAGCT19253.4846633E-45.7038751
CTTGAAT42652.0008883E-115.6637541
AGCACAC24451.1626962E-55.5813855
CTCCCAT32804.7333742E-85.57924751
CTCACTT18400.00121290775.56954431
CAACGAG25001.228182E-55.56317951
ATCACGA26355.0175004E-65.55595641
Produced by FastQC (version 0.11.5)