FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026952
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAAT4108.991372E-615.9210141
CTAACAT2950.004454128414.618793
CTCTAAC3100.00582927214.0378841
CCCACAT7601.3576755E-712.4062861
CCCATAT7902.9051644E-611.0170721
CCCAATT5952.9583578E-410.9707831
CTCTAAA4900.009684510510.3612951
CCAATCT8701.024011E-59.9138923
CTCTCAT5900.00339113929.8344491
GTTAAAT5950.00363628529.7518071
CTCCAAT10508.288298E-79.6705421
CGACAAT10801.2168857E-69.4019161
CTTCGGA10306.7358014E-58.4499881
CTCAAAT8609.0369064E-48.4336121
CCCAGCT9653.089046E-48.2675621
ATTCGCT7900.00395245438.2628041
CGATAAT11653.5831796E-58.0204664
CTCATAT9250.0018566867.840981
CTCCGTT9400.00217485177.71585751
CACAAAG11352.0823466E-47.66827151
Produced by FastQC (version 0.11.5)