FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67
Sequences flagged as poor quality0
Sequence length137-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCACGAATATTGATATTCATGCTGGTATTAAGGTCACCCCCACTTGAAC11.4925373134328357No Hit
ATCAAAAGGCAATGTTGGAGGAGTTCGCCGCACAGCAGGGCTTTACAAAC11.4925373134328357No Hit
TTTCCGCTAAGGTAGAAGGTTTCTTAGGGAACGAGTTGCACGAATTAAAT11.4925373134328357No Hit
ATAAAATTCGTCCATTTATTTTTTCTCCAGATTCATGATTGCCGGAATGA11.4925373134328357No Hit
CGTTGGACAGGGCTTCGATAATCGTACCGTCCCTTTCAATAGCAGCCTGT11.4925373134328357No Hit
CAACAAATTCATAATGATTCTCATTGATAATATTCTGTTTGTTCACTGCA11.4925373134328357No Hit
ATTCGATGTGGCGGGGCGGACGGTCCAGGTGCAGCTCCTTTTGCATGTTG11.4925373134328357No Hit
GGTTCACCAAGCCAATACCGGTGCCTCCGCTATGTGTCAATTTCGGTTGG11.4925373134328357No Hit
CATCCAGCAGAGTACGACAGCCGCGATGCAGGTGAAAGAAAAAATTTTCA11.4925373134328357No Hit
CTGGCTCACCCGGAATCCTCAAATACAATATATAACGAGAGACCGTGGAA11.4925373134328357No Hit
TTTTGACAATTATTTTGAAAATCTTTGACAAAAATTTTGAGCGGCTACAA11.4925373134328357No Hit
CAATACATACCCGTTTAGGGTAAAAGACTTCTTGCTGGATTGTAGAGCGT11.4925373134328357No Hit
CTGTGTGAGAGTATAAGGGAAGAAATGTCCCTTGACCCGAGCGATGCGTC11.4925373134328357No Hit
CGGAGCTGTCTATTCCGCCACTCATTCCTACCAGGACTCTTTTATCTCTG11.4925373134328357No Hit
ACAGACGGCGCGGCTCTGCCTGAAAAAAGCCCACGCCACCGAAAAGGATG11.4925373134328357No Hit
CACATCTTTTTTAGTAACGCCTTTCAATACCAATCTGTTTACAAAATCAG11.4925373134328357No Hit
CTCCCAACATTTCGCGGACGTCCGGCGTTTCATCCTGGTCGTGCGCATTC11.4925373134328357No Hit
CTTGTTTACCAGTACCCGGTTGTTTCCCTGATGGTCCTTCAGGCAATAGT11.4925373134328357No Hit
CCACTTCTTTCCAACCACGAAGAGATTTTTCCACTAATACCTGCGGAGAG11.4925373134328357No Hit
ATGTTTACAAGCCTTATTTGAGTTTAACCTGTTAAGACACTACGGAGGCT11.4925373134328357No Hit
GGTCAGCCTTTCCAGAAACCTGTGAATCGACTTCGGCGAGGGGACGGGAG11.4925373134328357No Hit
CTTAGCCCATCCCATAGGCGTTTCTTCAAACCCATCCATATATTCAATTG11.4925373134328357No Hit
CCAGACAGCCCAGCAGATGCTGGACCGTGAACAGAACCGCCCCGACGGGG11.4925373134328357No Hit
AGACAAAGGCATCATAGCGGTTGAAGTTTTCCGCTGTGAATTTGCCGGCG11.4925373134328357No Hit
GGCAAAACACCATTATTCTCCAACAAGACGATAGACTGGCTGGCAATCTC11.4925373134328357No Hit
CTATCAGGAGGCGGGCCGCGCGGGCCGCGACGGTCTGCCGTCCCGCTGCA11.4925373134328357No Hit
CATCTATCAGACCATTTTGCGGGCACTGCCAGACATCATGCTCGCAGACC11.4925373134328357No Hit
CTCCCTTTTCCAATCCGAGCGTGGCAGCAGAAAGAAAGTCCATGAAGCCT11.4925373134328357No Hit
GGACAGGAACTGCGGCACGGTGATGGCTTCCAGATTGGCCGAATACCGCC11.4925373134328357No Hit
GCCCCCAGTTGATTGAGCCGATGATGCTCAGAATCAGCGCAAGCATGTCC11.4925373134328357No Hit
GATAATGAGTTTGCTGCAAAGGATTTTACAGCTTTGAGTCCTGAATTGCA11.4925373134328357No Hit
GACATGAATAAATGGCTTGTAAAAAGTGCTATACACATTTTCCACAGCCG11.4925373134328357No Hit
CCGGAATTGTAGTTTGTAGAGTTGAACATAGAGAAGCCTGAGATTTCTTC11.4925373134328357No Hit
CCAGACGGACAAGACTGCTGTGAAAATTGTTCCTCCACCGGGCGCGGCAA11.4925373134328357No Hit
CTTATCATAAACGGTGGTACATTTGCCGGAGGTCTTAATACCATTAAAAA11.4925373134328357No Hit
CCACGGCACAGCCCGTTTTCGAAGAGCGCACGCAGGTCTTCCCGGTAGTC11.4925373134328357No Hit
ACCATTTAATGGCAAGTACATTCTTGTCCGGATCGGTTACTGTATATTTA11.4925373134328357No Hit
CGCTCCTCCGTATACACGGGATTATGGCGTTACCTCTGAATCGTGCTTGC11.4925373134328357No Hit
CAGAGATTGATCCGCGCTTCCGGACTTATTACATTGCCGATGAGGTGATC11.4925373134328357No Hit
ATAATCATATACAAAACGGGAGCTGTGACCGGCTGAGATGGAGGTCGTCT11.4925373134328357No Hit
CAGACACCAGTTTCTCGTGCATCTGAGCGGCCAATACAACCGGGTGCATG11.4925373134328357No Hit
CAGTGAATCCGATTACGGCTTTAATGGCGTAGATATGGGCTCTTATCCTG11.4925373134328357No Hit
CTGGGTGATCGCAGAGGCACTGCGCAGAGGCGTAAGCTATGACAAGATCC11.4925373134328357No Hit
TGATGCTTTTCGCAGCACAATGAACAGTAAGGATTCAAAAGAAGAAACCA11.4925373134328357No Hit
CATGATTCCTATGACAGTGGTTACGGGCCTTTTAATGAGTAATCCGAATT11.4925373134328357No Hit
ACCGGATTTACCTTTTAACATATCGGATAAGGATTTTAATGCTCTGTTTC11.4925373134328357No Hit
AAAAACTGAATGCTTTGCAGAGAAGAAAAAACCTGGAAAAAGCGTTCTGC11.4925373134328357No Hit
CCATAAAGAACACAAGTTTATAATTTTATAATATAAGAAAAAGGATATGA11.4925373134328357No Hit
GGCTAAACATCAAAATCAGAAATACGAGTGGTATAACCATCATACAGATA11.4925373134328357No Hit
TACCGGTATCGTTCTTCCGAATACATGGCACCCATCTGGTTCAGGATTTT11.4925373134328357No Hit
TTACAATGGCCGCGGAAGAAAGCGGCATGAGCAGGGAGCTTTTTGAAAAA11.4925373134328357No Hit
CAGTGATAGAAGCCGGTATAAAAAGAGTATATGTGGGCTCGGATGACCCG11.4925373134328357No Hit
GTGTTGACAATAATGTTGCGCTTGTTTTCAACGATATCGAAATGGCGCCT11.4925373134328357No Hit
CAATCTTGTAACGTTGGGATTCTGTCGTACCATTTTATTATCTTTTTAAT11.4925373134328357No Hit
CTCTGTTCCTGCGAGAGAAATCTGCTCTGAAATTTCACTTGCGGGCATAA11.4925373134328357No Hit
TAGCCTCTTGGTAACGAACAGGCTCGATACGACGTTTCCATTTTTGGTCA11.4925373134328357No Hit
TAGTTGGCTGTCACATCCGCACCTGCAGCATTTTCAATCTTCACACCACT11.4925373134328357No Hit
CTGCTCTACATCGTCCTGCCGCAGGCCTACCGCAACATCGTGCCGACCCT11.4925373134328357No Hit
NACACTTTCGCCTCGTGGAACGTCAACGGCCTGCGCGCCGTGCTCAAAAA11.4925373134328357No Hit
TCAGCCCTTTTTTAAAAGGTTATAAGTTTTGGTACTCATCATCGTCTCTT11.4925373134328357No Hit
GATTACGGAAAGTCAAAGAGAGCGTGCTGAGAGTCTTGGATATACAGTTG11.4925373134328357No Hit
GTGTAAAACTCTTGTAGTTGCTGTATACTTGCAAACTCGTAAAACGTCGG11.4925373134328357No Hit
ATTCGTCAGAACCGAGAGTTTTTTAATGTTGCACCACAAAAAGCACTGGA11.4925373134328357No Hit
CCTTGTTCCCGCGCGCATTTATCTCCAAGCTGTCCGATGCTCCCTTTACA11.4925373134328357No Hit
ATTCAGACCGTGCCCGATGCTGAACAAAGCCGAAGACACCAGCTGCAGTT11.4925373134328357No Hit
GTCGTGGCTTCCGACACCGACGAACTGGAGGGGCGTTTCCCCACCGGCGA11.4925373134328357No Hit
CCGCCATAAACTAAGCAACAGGATTTCCACAGGTAACCGGAGCGGTTTTC11.4925373134328357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTA50.0140.02
TTACCAG50.0140.06
CCAGTAC50.0140.09
GTCGTCC50.0140.0140
TGTTTAC50.0140.03
GTTTACC50.0140.04
CTTGTTT50.0140.01
TTTACCA50.0140.05
TACCAGT50.0140.07
ACCAGTA50.0140.08
Produced by FastQC (version 0.11.5)