FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585622
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGGA3100.005459564714.1758181
ACTATTC4302.4772598E-413.4390045
CTCAAAC4200.00285280412.2069551
CTCCGCT4856.085834E-412.081111
CACCCTG4650.006100482311.0256381
CTCATTG4950.00970287710.3574171
GGATGAT8506.1890696E-610.3400091
CCTATTT6000.00359014849.7655641
CTTTAAT8709.183116E-48.418591
TCAGATT7750.003438988.3899732
CATTAAA8550.00735637647.70965621
CTTCAAA11150.00130256827.22564171
CAAAAGA12300.0036650716.55007361
Produced by FastQC (version 0.11.5)