FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985857
Sequences flagged as poor quality0
Sequence length100-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT7903.385172E-610.87885959
CACACGT6154.5166837E-410.4808527
AGCACAC10750.00112455797.3284715
CAAATGT11903.91678E-47.25993821
ACACGTC10350.00630003966.9197258
GAGCACA11750.00287808436.7047724
TAAAGTT13500.00174225526.3672693
AGAGCAC12700.00646974946.2043273
GTTCGAT21851.1007063E-55.9308871
CCTTGAT30257.2168405E-85.7119511
CCAAAAT18400.00149508125.47783331
CAAAGAT24557.075685E-55.27861071
AACAATT19450.0031859595.15757372
Produced by FastQC (version 0.11.5)