FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603459
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAG1950.006993679318.5171071
TAGCCAG2000.00821802118.0062714
CCCACCT2901.9204423E-417.4316231
CCTCGGA3750.001353003113.48045351
CTCGGAA4200.003170704212.0361191
CTTAAAT5852.6282086E-411.1102651
TTATGGT6053.6768088E-410.7144763
CCATGTT5950.00376454979.7098121
CCTGTAT6350.0064352669.0981691
CAATGAT8558.8888046E-48.44641
CTTTTAT11653.3690056E-58.0585181
CTTTGAT9450.0023868227.6419811
CTTCAAA11352.1843553E-47.63524771
ATTTTCC15401.997352E-57.015436
AACTTTT10550.00716159546.8270223
CTTTCTT15800.0091436835.4848141
CTTCTTT20107.443409E-45.38930751
Produced by FastQC (version 0.11.5)