FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951352
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3250.032.924617
CACGTCT4000.028.534669
ACACGTC4200.027.1758698
AGCACAC6901.0913936E-1116.5444415
GCACACG6801.4733814E-1015.7385086
AGAGCAC7203.4924597E-1014.8641473
GAGCACA8051.8608262E-913.2946414
GTTTGTT9955.069815E-47.8951781
AAGAGCA15453.015899E-67.38955642
GAAGAGC14551.0163174E-57.362421
GTTTGAT10050.00486252837.1060171
AGTCACC4403.1789023E-76.48528620-24
CAGTCAC4209.823161E-76.454404420-24
Produced by FastQC (version 0.11.5)