FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451297
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGCG15401.51083605E-57.15933751
CTCGTTT17852.225108E-67.00024131
CATTAAC13651.9380984E-47.00024031
CTTAAAT22202.1762935E-76.62184951
CCCTTAT15601.3773632E-46.59638071
CTTTGAT41900.06.49067641
CCCCGAT13850.00173905286.368451
CCCAGTT15156.907882E-46.30714751
CTCCGAT18853.698276E-56.2389411
CCCCGCT13000.0063096396.21944431
CTTTTAG13550.0096893695.9669951
CCCACAA17100.00294515825.58791161
CTCAAAT22608.476023E-55.5289511
CTTGCAT20005.5194495E-45.51268961
CCTGGAT17350.00349723525.5073941
CATCAAT41502.9831426E-105.49055051
GATCAAT17450.00374297085.4758331
CCTGATT30351.7910315E-65.3280251
TTAACAT19350.0027639915.21667583
CCTCTGT21250.00124278515.18841361
Produced by FastQC (version 0.11.5)