FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1409049
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT4500.023.8560547
ACACGTC4550.023.5938998
CACGTCT5550.020.6322659
GCACACG9003.6379788E-1214.3136336
AGAGCAC8905.802576E-1012.8639053
GAGCACA11603.279638E-910.4888114
AGCACAC10851.4641228E-79.8942175
AGCGACG7600.00314444168.4751776
CCTAAAA8900.0014170228.0593881
GAAGAGC16207.059898E-77.5270711
CTTGGAT9650.00313796857.4330111
CACCAAT12150.00402029846.49394371
CAATCAT18153.0355737E-56.3231791
CCCGAAA12900.00749829976.1163891
AAGAGCA21004.152356E-55.7944362
ATATTGC23203.1646196E-55.55270246
CATAAAT19605.835561E-45.48943041
Produced by FastQC (version 0.11.5)