FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences790371
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGTC7853.6476908E-49.2364651
CTTCAGA7100.00150439089.1909341
CCCAAAA8054.6915314E-49.0069891
AAGCTTG8155.477605E-48.8683095
GGATTTA9201.9129194E-48.6417824
TAAGGAT8100.00510569438.0307463
CATTCAG8150.00524442648.0068251
CACAAAG9250.00186129157.83851431
CTCAAAG8650.0089398347.5440041
CTTCTAT10608.4773585E-47.52423431
CTCAAAA11803.2694318E-47.3735181
CTTTGAT13101.3779663E-47.1952771
CTTTAAA14456.02395E-57.0248271
ATCAGAT12100.0034349116.5914781
CTTATTT13050.00754882636.11163851
CTTTTAT19205.8726575E-56.04218771
ATTTAAA18752.8843185E-45.78213746
TCAAAGA15150.00569317545.7259762
ATTTCAA19304.0883076E-45.63520151
CAAAAGA18800.00178842885.3994021
Produced by FastQC (version 0.11.5)